Rabbit Anti-Human NGFB (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01138 |
| Gene Id | 4803 |
| Gene Symbols | NGF |
| Protein Name / Synonyms | NGFB (Center) |
| Clonality | Polyclonal |
| Preparation | This NGFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-115 amino acids from the Central region of human NGFB. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NGFB is a member of the NGF-beta family. It is a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in the gene encoding NGFB have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the gene's expression is associated with allergic rhinitis.
Specificity
Other Names
NGF; NGFB; Beta-nerve growth factor
NCBI Accession #
NP_002497.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
26959
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of NGFB Antibody (Center) (Cat# 102-17614) in NCI-H460 cell line lysates (35ug/lane). NGFB (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with NGFB antibody (Center) (Cat# 102-17614), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | NGFB Antibody (Center) (Cat# 102-17614) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of NGFB Antibody (Center)(Cat# 102-17614) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.