NPR-C antibody [N3C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Chicken, Human |
| Host Species | Rabbit |
| Gene Id | 4883 |
| Protein Name / Synonyms | NPR-C |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ANPRC antibody, C5orf23 antibody, FLJ14054 antibody, GUCY2B antibody, MGC22189 antibody, NPRC antibody, NPR3 antibody, atrial natriuretic peptide receptor 3 antibody, atrial natriuretic peptide receptor type C antibody, atrial natriuretic peptide clearance receptor antibody, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
Specificity
Species Reactivity
Human, Chicken
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
60 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 265 and 514 of NPR-C
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
NPR-C antibody [N3C3] detects NPR-C protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: NPR-C protein stained by NPR-C antibody [N3C3] (107-10890) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: NPR-C protein stained by NPR-C antibody [N3C3] (107-10890) diluted at 1:500.
Blue: Hoechst 33342 staining.
NPR-C antibody [N3C3] detects NPR-C protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
NPR-C antibody [N3C3] (107-10890) dilution: 1:500.
Sample: Paraffin-embedded mouse testis.
NPR-C antibody [N3C3] (107-10890) dilution: 1:500.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPR-C (107-10890) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NPR-C (107-10890) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: Hep G2
7.5% SDS PAGE
107-10890 diluted at 1:10000
A: Hep G2
7.5% SDS PAGE
107-10890 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.