Rabbit Anti-Human NRAS (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P01111 |
| Gene Id | 4893 |
| Gene Symbols | NRAS |
| Protein Name / Synonyms | NRAS (C-term) |
| Clonality | Polyclonal |
| Preparation | This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-179 amino acids from the C-terminal region of human NRAS. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting |
| Research Area | Apoptosis, Cardiovascular Disease, Cancer, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
Specificity
Predicted Reactivity
Pig
Other Names
NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
NCBI Accession #
NP_002515.1
Other Accession #
Q2MJK3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
21229
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | NRAS Antibody (C-term) (Cat# 102-17599) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NRAS antibody detected the NRAS protein (arrow). |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | NRAS Antibody (C-term) (Cat# 102-17599) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.