Synonyms
4933414E04Rik antibody, FLJ23743 antibody, NOPD1 antibody, NSUN6 antibody, NOL1/NOP2/Sun and PUA domain-containing protein 1 antibody, putative methyltransferase NSUN6 antibody, NOL1/NOP2/Sun domain family member 6 antibody, nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 antibody, NOL1/NOP2/Sun domain family 6 antibody, "NOL1/NOP2/Sun domain family, member 6 antibody", "NOP2/Sun domain family, member 6 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 179 and 469 of NSUN6 (Uniprot ID# Q8TEA1)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NSUN6(107-10671) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:293T
10% SDS PAGE
107-10671 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.