Nucleoporin p62 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 23636 |
| Protein Name / Synonyms | Nucleoporin p62 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp547L134 antibody, FLJ20822 antibody, FLJ43869 antibody, IBSN antibody, MGC841 antibody, SNDI antibody, p62 antibody, NUP62 antibody, 62 kDa nucleoporin antibody, nucleoporin Nup62 antibody, nuclear pore glycoprotein p62 antibody, nucleoporin 62kDa antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
53 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 230 and 490 of Nucleoporin p62
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Nucleoporin p62 antibody immunoprecipitates NUP62 protein in IP experiments.
IP samples: 293T whole cell extract
A. Control with 4 µg of preimmune Rabbit IgG
B. Immunoprecipitation of NUP62 protein by 4 µg Nucleoporin p62 antibody (107-10720)
7.5 % SDS-PAGE
The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody (107-10720) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
IP samples: 293T whole cell extract
A. Control with 4 µg of preimmune Rabbit IgG
B. Immunoprecipitation of NUP62 protein by 4 µg Nucleoporin p62 antibody (107-10720)
7.5 % SDS-PAGE
The immunoprecipitated NUP62 protein was detected by Nucleoporin p62 antibody (107-10720) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
7.5% SDS PAGE
107-10720 diluted at 1:5000
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
7.5% SDS PAGE
107-10720 diluted at 1:5000
Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
107-10720 diluted at 1:5000
A: PC-12
7.5% SDS PAGE
107-10720 diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using nucleoporin p62 (107-10720) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A:Hep G2
B:MOLT4
C:Raji
10% SDS PAGE
107-10720 diluted at 1:5000
A:Hep G2
B:MOLT4
C:Raji
10% SDS PAGE
107-10720 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.