NuMA antibody [N1], N-Terminus, 0.42 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 4926 |
| Protein Name / Synonyms | NuMA, N-term |
| Concentration (lot specific) | 0.42 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
NUMA antibody, NUMA1 antibody, nuclear mitotic apparatus protein 1 antibody, centrophilin stabilizes mitotic spindle in mitotic cells antibody, SP-H antigen antibody, structural nuclear protein antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
238 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID# Q14980)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:2000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA(107-11070) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg A375 whole cell lysate/extract
5 % SDS-PAGE
NuMA antibody [N1], N-term (107-11070) dilution: 1:1000
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg A375 whole cell lysate/extract
5 % SDS-PAGE
NuMA antibody [N1], N-term (107-11070) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA (107-11070) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.