O-GlcNAc transferase antibody [N1N2], N-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 8473 |
| Protein Name / Synonyms | O-GlcNAc transferase, N-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Post-Translational Modifications, Glycosylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ23071 antibody, HRNT1 antibody, MGC22921 antibody, O-GLCNAC antibody, OGT antibody, O-GlcNAc transferase p110 subunit antibody, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody, O-GlcNAc transferase subunit p110 antibody, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody, O-linked N-acetylglucosamine transferase 110 kDa subunit antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
117 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 280 of O-GlcNAc transferase (Uniprot ID# O15294)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution: 1:500.
Sample: Paraffin-embedded U373 xenograft.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution: 1:500.
O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution:
Sample: Paraffin-embedded mouse liver.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution:
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10902 diluted at 1:1000
A: Mouse brain
5% SDS PAGE
107-10902 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-10902 diluted at 1:1000
A: Hela
7.5% SDS PAGE
107-10902 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.