Rabbit Anti-Human OPN-a/b (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P10451 |
| Gene Id | 6696 |
| Gene Symbols | SPP1 |
| Protein Name / Synonyms | OPN-a/b (N-term) |
| Clonality | Polyclonal |
| Preparation | This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b. |
| Concentration (lot specific) | 0.280 mg/ml |
| Recommended Applications | Western Blotting, IF, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
Specificity
Other Names
SPP1; BNSP; OPN; Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; Urinary stone protein; Uropontin
NCBI Accession #
NP_000573.1;NP_001035147.1;NP_001035149.1;NP_001238758.1;NP_001238759.1
Other Accession #
NP_000573.1; NP_001035147.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35423
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | Western blot analysis of OPN-a/b (arrow) using rabbit polyclonal OPN-a/b Antibody (N-term) (Cat# 102-11153). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the OPN-a/b gene. |
 | OPN-a/b Antibody (N-term) (Cat# 102-11153)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OPN-a/b Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of OPN-a/b Antibody (N-term)(Cat# 102-11153) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.