p63 antibody [N2C1], Internal, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8626 |
| Protein Name / Synonyms | p63, Internal |
| Concentration (lot specific) | 0.34 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AIS antibody, B(p51A) antibody, B(p51B) antibody, EEC3 antibody, KET antibody, LMS antibody, NBP antibody, OFC8 antibody, RHS antibody, SHFM4 antibody, TP53CP antibody, TP53L antibody, TP73L antibody, p40 antibody, p51 antibody, p53CP antibody, p63 antibody, p73H antibody, p73L antibody, TP63 antibody, keratinocyte transcription factor KET antibody, CUSP antibody, amplified in squamous cell carcinoma antibody, tumor protein p53-competing protein antibody, tumor protein p63 deltaN isoform delta antibody, chronic ulcerative stomatitis protein antibody, tumor protein 63 antibody, transformation-related protein 63 antibody, tumor protein p63 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
77 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 125 and 415 of p63 (Uniprot ID# Q9H3D4)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(107-10336) antibody at 1:100 dilution.
p63 antibody detects p63 protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
7.5 % SDS-PAGE
p63 antibody (107-10336) dilution: 1:500
A. 30 µg A431 whole cell lysate/extract
7.5 % SDS-PAGE
p63 antibody (107-10336) dilution: 1:500
p63 antibody [N2C1], Internal detects TP63 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500
p63 antibody [N2C1], Internal detects TP63 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500
Immunofluorescence analysis of paraformaldehyde-fixed A431, using p63 (107-10336) antibody at 1:200 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.