PAD4 antibody, 0.73 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 23569 |
| Protein Name / Synonyms | PAD4 |
| Concentration (lot specific) | 0.73 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), ChIP assay, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
PAD antibody, PAD4 antibody, PADI5 antibody, PDI4 antibody, PDI5 antibody, PADI4 antibody, peptidyl arginine deiminase, type V antibody, PADI-H protein antibody, protein-arginine deiminase type IV antibody, HL-60 PAD antibody, protein-arginine deiminase type-4 antibody, peptidyl arginine deiminase, type IV antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
74 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 326 and 611 of PAD4 (Uniprot ID# Q9UM07)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),ChIP assay,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, ChIP. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PADI4 antibody immunoprecipitates PADI4 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 ?g preimmune rabbit IgG
B. 5 ?g of PADI4 antibody (107-11100)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
B. 5 ?g of PADI4 antibody (107-11100)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAD4(107-11100) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PAD4(107-11100) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
7.5% SDS PAGE
107-11100 diluted at 1:1000
A: Hela
B: Hep G2
7.5% SDS PAGE
107-11100 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.