Rabbit Anti-Human PAK3 (Center K218) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O75914 |
| Gene Id | 5063 |
| Gene Symbols | PAK3 |
| Protein Name / Synonyms | PAK3 (Center K218) |
| Clonality | Polyclonal |
| Preparation | This PAK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-247 amino acids from the Central region of human PAK3. |
| Concentration (lot specific) | 1.640 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PAK3, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. PAK3 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. It shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK. This protein is highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. PAK3 is autophosphorylated when activated by CDC42/p21. Defects in PAK3 are the cause of non-specific X-linked nonsyndromic mental retardation type 30 (MRX30). The protein structure contains 1 CRIB domain.
Specificity
Other Names
PAK3; OPHN3; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3
NCBI Accession #
NP_001121638.1;NP_001121639.1;NP_001121640.1;NP_001121644.1;NP_001121645.1;NP_002569.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
62310
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of PAK3(arrow) using rabbit polyclonal PAK3 Antibody (Cat# 102-17703). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK3 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.