Rabbit Anti-Human PCK2 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16822 |
| Gene Id | 5106 |
| Gene Symbols | PCK2 |
| Protein Name / Synonyms | PCK2 (N-term) |
| Clonality | Polyclonal |
| Preparation | This PCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human PCK2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
Specificity
Other Names
PCK2; PEPCK2; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; Phosphoenolpyruvate carboxylase
NCBI Accession #
NP_001018083.1;NP_004554.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
70730
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | Western blot analysis of anti-PCK2 Antibody (N-term) (Cat# 102-17798) in HepG2 cell line lysates (35ug/lane). PCK2 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Western blot analysis of PCK2 (arrow) using rabbit polyclonal PCK2 Antibody (Q39) (Cat# 102-17798). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCK2 gene. |
 | Confocal immunofluorescent analysis of PCK2 Antibody (N-term)(Cat# 102-17798) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.