Synonyms
DKFZp781I24156 antibody, PCNP antibody, PEST proteolytic signal-containing nuclear protein antibody, PEST-containing nuclear protein antibody, PEST proteolytic signal containing nuclear protein antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
19 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 50 of PCNP (Uniprot ID# Q8WW12)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using PCNP(107-11001) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
12% SDS PAGE
107-11001 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.