Synonyms
HCAM1 antibody, HSPDE1A antibody, MGC26303 antibody, PDE1A antibody, calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase antibody, "3',5' cyclic nucleotide phosphodiesterase antibody", "calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A antibody", phosphodiesterase-1A antibody, hCam-1 antibody, calmodulin-dependent phosphodiesterase antibody, cam-PDE 1A antibody, 61 kDa Cam-PDE antibody, "phosphodiesterase 1A, calmodulin-dependent antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
61 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 212 and 535 of PDE1A (Uniprot ID# P54750)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using PDE1A(107-10981) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
7.5% SDS PAGE
107-10981 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.