PDE4D antibody [C3], C-Terminus, 0.3 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5144 |
| Protein Name / Synonyms | PDE4D, C-term |
| Concentration (lot specific) | 0.3 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp686M11213 antibody, DPDE3 antibody, FLJ97311 antibody, HSPDE4D antibody, PDE4DN2 antibody, STRK1 antibody, PDE4D antibody, "phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) antibody", cAMP-specific phosphodiesterase PDE4D6 antibody, "cAMP-specific 3',5'-cyclic phosphodiesterase 4D antibody", dunce-like phosphodiesterase E3 antibody, "phosphodiesterase 4D, cAMP-specific antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
91 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 746 and 809 of PDE4D (Uniprot ID# Q08499)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D(107-10469) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.
Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D(107-10469) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
7.5% SDS PAGE
107-10469 diluted at 1:1000
A: A431
B: HeLa
7.5% SDS PAGE
107-10469 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.