Synonyms
FLJ90181 antibody, HSPDE9A2 antibody, PDE9A antibody, phosphodiesterase PDE9A21 antibody, "CGMP-specific 3',5'-cyclic phosphodiesterase type 9 antibody", "high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A antibody", phosphodiesterase 9A antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
68 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 216 of PDE9A (Uniprot ID# O76083)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PDE9A(107-10798) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10798 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
107-10798 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.