Rabbit Anti-Human PDGFRB (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P09619 |
| Gene Id | 5159 |
| Gene Symbols | PDGFRB |
| Protein Name / Synonyms | PDGFRB (N-term) |
| Clonality | Polyclonal |
| Preparation | This PDGFRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-72 amino acids from the N-terminal region of human PDGFRB. |
| Concentration (lot specific) | 1.200 mg/ml |
| Recommended Applications | IHC-P, IF, FC |
| Research Area | Angiogenesis, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses the gene to that of the translocation, ETS, leukemia gene, results in chronic myelomonocytic leukemia.
Specificity
Other Names
PDGFRB; PDGFR; PDGFR1; Platelet-derived growth factor receptor beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; CD_antigen=CD140b; Flags: Precursor
NCBI Accession #
NP_002600.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
123968
Recommended Dilutions
IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50; WB: 1:1000
 | Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of PDGFRB Antibody (N-term)(Cat# 102-17537) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of anti-PDGFRB Antibody (N-term) (Cat# 102-17537) in cell line lysates (35ug/lane). PDGFRB(arrow) was detected using the purified Pab). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.