PEX19 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5824 |
| Protein Name / Synonyms | PEX19 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
D1S2223E antibody, FLJ55296 antibody, HK33 antibody, PMP1 antibody, PMPI antibody, PXF antibody, PXMP1 antibody, PEX19 antibody, peroxisomal biogenesis factor 19 antibody, peroxisomal farnesylated protein antibody, housekeeping gene, 33kD antibody, 33 kDa housekeeping protein antibody, peroxin-19 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 299 of PEX19 (Uniprot ID# P40855)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PEX19(107-10933) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: Raji
B: K562
C: THP-1
D: NCI-H929
12% SDS PAGE
107-10933 diluted at 1:10000
A: Raji
B: K562
C: THP-1
D: NCI-H929
12% SDS PAGE
107-10933 diluted at 1:10000
Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
107-10933 diluted at 1:10000
A: mouse liver
12% SDS PAGE
107-10933 diluted at 1:10000
PEX19 antibody detects PEX19 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
PEX19 antibody (107-10933) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
PEX19 antibody (107-10933) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PEX19(107-10933) antibody at 1:500 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.