Rabbit Anti-Human Phospho-CCNB1(S35) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14635 |
| Gene Id | 891 |
| Gene Symbols | CCNB1 |
| Protein Name / Synonyms | Phospho-CCNB1(S35) |
| Clonality | Polyclonal |
| Preparation | This CCNB1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S35 of human CCNB1. |
| Concentration (lot specific) | 0.290 mg/ml |
| Recommended Applications | Dot Blotting, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].
Specificity
Other Names
CCNB1; CCNB; G2/mitotic-specific cyclin-B1
NCBI Accession #
NP_114172.1
Other Accession #
NP_114172.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
48337
Recommended Dilutions
WB: 1:1100; DB: 1:500
 | Western blot analysis of lysate from Hela cells(from left to right),untreated or treated with Nocodazole at 50ng/ml,using Phospho-CCNB1-S35 Antibody (Cat# 102-15729) or CCNB1-S9 Antibody.Lysate at 15ug per lane.102-15729 was diluted at 1:1100 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. |
 | Dot blot analysis of CCNB1 Antibody (Phospho S35) Phospho-specific Pab (Cat# 102-15729) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.