Rabbit Anti-Human Phospho-CDC25A(T507) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P30304 |
| Gene Id | 993 |
| Gene Symbols | CDC25A |
| Protein Name / Synonyms | Phospho-CDC25A(T507) |
| Clonality | Polyclonal |
| Preparation | This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T507 of human CDC25A. |
| Concentration (lot specific) | 0.460 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Specificity
Predicted Reactivity
Mouse, Rat, Bovine
Other Names
CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A
NCBI Accession #
NP_001780.2;NP_963861.1
Other Accession #
P48965; P48964; A7MBD1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
59087
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | The anti-Phospho-CDC25A-T507 Pab (Cat# 102-15282) is used in Western blot to detect Phospho-CDC25A-T507 in cells transfected with wild type or mutant T507 A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.