Rabbit Anti-Human Phospho-EGFR(Y1172) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P00533 |
| Gene Id | 1956 |
| Gene Symbols | EGFR |
| Protein Name / Synonyms | Phospho-EGFR(Y1172) |
| Clonality | Polyclonal |
| Preparation | This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1172 of human EGFR. |
| Concentration (lot specific) | 0.410 mg/ml |
| Recommended Applications | Western Blotting, Dot Blotting |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. EGFR is a receptor for EGF, but also for other members of the EGF family, including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Specificity
Other Names
EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
NCBI Accession #
NP_005219.2;NP_958439.1;NP_958440.1;NP_958441.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
134277
Recommended Dilutions
WB: 1:1000; DB: 1:500
 | Western blot analysis of extracts from A431 cells, untreated or treated with EGF, using phospho EGFR-Y1172 (left) or ErBB2 antibody (right) |
 | Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2). |
 | Dot blot analysis of anti-EGFR-pY1172 Phospho-specific Pab (Cat# 102-15412) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
 | Western blot analysis of extracts from A431 cells,untreated or treated with EGF using Phospho-EGFR(Tyr1172)(left) or EGFR antibody(right). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.