Rabbit Anti-Human Phospho-EP300(S89) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q09472 |
| Gene Id | 2033 |
| Gene Symbols | EP300 |
| Protein Name / Synonyms | Phospho-EP300(S89) |
| Clonality | Polyclonal |
| Preparation | This EP300 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S89 of human EP300. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | Dot Blotting, IHC-P |
| Research Area | Cardiovascular Disease, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
Specificity
Predicted Reactivity
Mouse
Other Names
EP300; P300; Histone acetyltransferase p300; E1A-associated protein p300
NCBI Accession #
NP_001420.2
Other Accession #
B2RWS6
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
264161
Recommended Dilutions
DB: 1:500; IHC: 1:50-100
 | Dot blot analysis of anti-Phospho-EP300-S89 Pab (Cat# 102-15333) on nitrocellulose membrane. 50ng of Phospho-peptide (BP3197a) or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.