Rabbit Anti-Human Phospho-HER4(Y1162) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q15303 |
| Gene Id | 2066 |
| Gene Symbols | ERBB4 |
| Protein Name / Synonyms | Phospho-HER4(Y1162) |
| Clonality | Polyclonal |
| Preparation | This HER4 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1162 of human HER4. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IHC-P, Western Blotting, IF |
| Research Area | Cardiovascular Disease, Cancer, HER/ErbB Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
HER4/ERBB4 is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. It is a receptor for NDF/heregulin.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ERBB4; HER4; Receptor tyrosine-protein kinase erbB-4; Proto-oncogene-like protein c-ErbB-4; Tyrosine kinase-type cell surface receptor HER4; p180erbB4; ERBB4 intracellular domain; s80HER4
NCBI Accession #
NP_001036064.1;NP_005226.1
Other Accession #
Q62956; Q61527
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
146808
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | Phospho-HER4-Y1162 Antibody (cat# 102-15306)was used to detect Phospho-HER4-Y1162 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50ng/ml) for 15 min Phospho-HER4-Y1162 Antibody (cat# 102-15306) was used at 1:750 in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Confocal immunofluorescent analysis of Phospho-HER4-Y1162 Antibody(Cat# 102-15306) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.