Rabbit Anti-Human Phospho-Nanog(S71) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9H9S0 |
| Gene Id | 79923 |
| Gene Symbols | NANOG |
| Protein Name / Synonyms | Phospho-Nanog(S71) |
| Clonality | Polyclonal |
| Preparation | This Nanog Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S71 of human Nanog. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | IF, Dot Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
Specificity
Predicted Reactivity
Monkey
Other Names
NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog
NCBI Accession #
NP_079141.2
Other Accession #
Q6NSW7; Q5TM84
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34620
Recommended Dilutions
IF: 1:10-50; DB: 1:500
 | Fluorescent confocal image of SY5Y cells stained with 102-15503 Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-15503 Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells. |
 | Dot blot analysis of anti-Phospho-Nanog-S71 Antibody (Cat# 102-15503) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.