Rabbit Anti-Human Phospho-Rb(S811) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06400 |
| Gene Id | 5925 |
| Gene Symbols | RB1 |
| Protein Name / Synonyms | Phospho-Rb(S811) |
| Clonality | Polyclonal |
| Preparation | This Rb Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S811 of human Rb. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Dot Blotting, IHC-P, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression occurs in some cases.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; pp110
NCBI Accession #
NP_000312.2
Other Accession #
P33568; P13405
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
106159
Recommended Dilutions
DB: 1:500; IHC: 1:50-100; IF: 1:10-50
 | Dot blot analysis of anti-hRb-S811 Phospho-specific Pab (Cat# 102-15350) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
 | Confocal immunofluorescent analysis of Phospho-Rb-S811 Antibody(Cat# 102-15350) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
