Rabbit Anti-Human phospho-Sox2(S246) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48431 |
| Gene Id | 6657 |
| Gene Symbols | SOX2 |
| Protein Name / Synonyms | phospho-Sox2(S246) |
| Clonality | Polyclonal |
| Preparation | This Sox2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human Sox2. |
| Concentration (lot specific) | 0.450 mg/ml |
| Recommended Applications | IF, Dot Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
Specificity
Predicted Reactivity
Mouse
Other Names
SOX2; Transcription factor SOX-2
NCBI Accession #
NP_003097.1
Other Accession #
P48432; NP_003097.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34310
Recommended Dilutions
IF: 1:200; DB: 1:500
 | Fluorescent confocal image of SY5Y cells stained with phospho-Sox2- S246 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-15551 phospho-Sox2- S246 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the phospho-Sox2 immunosignal mainly to the nucleus. |
 | Dot blot analysis of anti-phospho-Sox2-pS246 Phospho-specific Pab (Cat# 102-15551) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.