Rabbit Anti-Human PI3KCA (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42336 |
| Gene Id | 5290 |
| Gene Symbols | PIK3CA |
| Protein Name / Synonyms | PI3KCA (C-term) |
| Clonality | Polyclonal |
| Preparation | This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1019-1050 amino acids from the C-terminal region of human PI3KCA. |
| Concentration (lot specific) | 1.6 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis, Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. This protein represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. PI3KCA has been found to be oncogenic and has been implicated in cervical cancers.
Specificity
Predicted Reactivity
Bovine
Other Names
PIK3CA; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA
NCBI Accession #
NP_006209.2
Other Accession #
P32871
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
124284
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:50-100
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Western blot analysis of anti-PI3KCA Pab (Cat# 102-17758) in HeLa cell lysate. PI3KCA (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
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PIK3CA Antibody (C-term) (Cat# 102-17758) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.