Synonyms
DKFZp686P05226 antibody, FLJ41892 antibody, p55 antibody, p55-GAMMA antibody, PIK3R3 antibody, ptdIns-3-kinase regulatory subunit p55-gamma antibody, ptdIns-3-kinase regulatory subunit gamma antibody, p55PIK antibody, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody, phosphoinositide-3-kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody, phosphatidylinositol 3-kinase regulatory subunit gamma antibody, PI3-kinase subunit p55-gamma antibody, PI3K regulatory subunit gamma antibody, 100% homology to SWISS-PROT Q92569 antibody, phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody, PI3-kinase regulatory subunit gamma antibody, phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) antibody, phosphoinositide-3-kinase, regulatory subunit 3 (gamma) antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
54 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 192 and 427 of PIK3R3
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PIK3R3(107-10711) antibody at 1:500 dilution.

PIK3R3 antibody detects PIK3R3 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
PIK3R3 antibody (107-10711) dilution: 1:1000

Sample (30µg whole cell lysate)
A:A431
7.5% SDS PAGE
107-10711 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.