PIM2 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 11040 |
| Protein Name / Synonyms | PIM2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
PIM2 antibody, proto-oncogene Pim-2 (serine threonine kinase) antibody, serine/threonine-protein kinase pim-2 antibody, serine/threonine protein kinase pim-2 antibody, pim-2h antibody, pim-2 oncogene antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
34 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 53 and 278 of PIM2 (Uniprot ID# Q9P1W9)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Breast ca, using PIM2(107-11098) antibody at 1:250 dilution.
PIM2 antibody detects PIM2 protein by Western blot analysis.
A. 50 µg mouse testis lysate/extract
12 % SDS-PAGE
PIM2 antibody (107-11098) dilution: 1:500
A. 50 µg mouse testis lysate/extract
12 % SDS-PAGE
PIM2 antibody (107-11098) dilution: 1:500
Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
107-11098 diluted at 1:1000
A: 293T
10% SDS PAGE
107-11098 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.