Rabbit Anti-Human PITX2 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q99697 |
| Gene Id | 5308 |
| Gene Symbols | PITX2 |
| Protein Name / Synonyms | PITX2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids from the C-terminal region of human PITX2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Pilx2 is a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. It plays a role in the terminal differentiation of somatotroph and lactotroph cell phenotypes, is involved in the development of the eye, tooth and abdominal organs, and acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin. Mutations in this protein are associated with Axenfeld-Rieger syndrome, iridogoniodysgenesis syndrome, and sporadic cases of Peters anomaly. A similar protein in other vertebrates is involved in the determination of left-right asymmetry during development.
Specificity
Predicted Reactivity
Mouse, Rat, Zebrafish, Chicken, Xenopus
Other Names
PITX2; ARP1; RGS; RIEG; RIEG1; Pituitary homeobox 2; ALL1-responsive protein ARP1; Homeobox protein PITX2; Paired-like homeodomain transcription factor 2; RIEG bicoid-related homeobox transcription factor; Solurshin
NCBI Accession #
NP_000316.2;NP_001191326.1;NP_001191327.1;NP_001191328.1;NP_700475.1;NP_700476.1
Other Accession #
Q9I8K3; Q6QU75; Q9PWR3; Q9R0W1; P97474; Q9W5Z2; O93385
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35370
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
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Fluorescent image of Hela cell stained with PITX2 Antibody (C-term)(Cat# 102-12417/SA070209B).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).PITX2 immunoreactivity is localized to Nucleus significantly. |
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Western blot analysis of anti-Pilx2 Pab (Cat# 102-12417) in CEM cell line lysates (35ug/lane). Pilx2(arrow) was detected using the purified Pab. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.