Rabbit Anti-Human PKC iota (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P41743 |
| Gene Id | 5584 |
| Gene Symbols | PRKCI |
| Protein Name / Synonyms | PKC iota (N-term) |
| Clonality | Polyclonal |
| Preparation | This PKC iota antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-207 amino acids from the N-terminal region of human PKC iota. |
| Concentration (lot specific) | 1.100 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PKC iota belongs to the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehydes-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis.
Specificity
Other Names
PRKCI; DXS1179E; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; nPKC-iota
NCBI Accession #
NP_002731.4
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
68262
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of PRKCI (arrow) using rabbit polyclonal PKC iota Antibody (N-term) (Cat# 102-17075). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCI gene (Lane 2) (Origene Technologies). |
 | Western blot analysis of hPKC-iota-C181 (Cat# 102-17075) in Hela cell line lysates (35ug/lane).PKC (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.