Rabbit Anti-Human PKC zeta (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q05513 |
| Gene Id | 5590 |
| Gene Symbols | PRKCZ |
| Protein Name / Synonyms | PKC zeta (N-term) |
| Clonality | Polyclonal |
| Preparation | This PKC zeta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 166-197 amino acids from the N-terminal region of human PKC zeta. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Inflammation, PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a constitutive kinase activity which is independent of calcium, and PKC activators, phosphatidylserine and diacylglycerol. Furthermore, it is insensitive to PKC inhibitors and cannot be activated by phorbol ester. The structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.
Specificity
Other Names
PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
NCBI Accession #
NP_001028753.1;NP_001028754.1;NP_001229803.1;NP_002735.3
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
67660
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of anti-PKCzeta Pab (Cat# 102-17080) in placenta lysate. PKCzeta (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PKC zeta antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.