Rabbit Anti-Human PKM2 (N-term E131) Antibody, 400 ul
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14618 |
| Gene Id | 5315 |
| Gene Symbols | PKM |
| Protein Name / Synonyms | PKM2 (N-term E131) |
| Clonality | Polyclonal |
| Preparation | This PKM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the N-terminal region of human PKM2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.
Specificity
Predicted Reactivity
Mouse, Rat, Rabbit
Other Names
PKM; OIP3; PK2; PK3; PKM2; Pyruvate kinase isozymes M1/M2; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Tumor M2-PK; p58
NCBI Accession #
NP_001193725.1;NP_001193726.1;NP_001193727.1;NP_001193728.1;NP_002645.3;NP_872270.1;NP_872271.1
Other Accession #
P11980; P11974; P52480
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
57937
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131) (Cat# 102-18132). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2) . |
 | PKM2 (N-term E131) Antibody (Cat# 102-18132) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated. |
 | PKM2 (N-term E131) Antibody (Cat# 102-18132) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.