PNPase antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 87178 |
| Protein Name / Synonyms | PNPase |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp762K1914 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, PNPT1 antibody, 3'-5' RNA exonuclease OLD35 antibody, polynucleotide phosphorylase-like protein antibody, PNPase 1 antibody, PNPase old-35 antibody, "polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody", polynucleotide phosphorylase 1 antibody, polyribonucleotide nucleotidyltransferase 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
86 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 25 and 251 (Q8TCS8) of PNPase
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Colon ca, using PNPase(107-11173) antibody at 1:250 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse liver
7.5% SDS PAGE
107-11173 diluted at 1:1000
A: Mouse liver
7.5% SDS PAGE
107-11173 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
107-11173 diluted at 1:1000
A: JurKat
7.5% SDS PAGE
107-11173 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.