PNPase antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 87178 |
| Protein Name / Synonyms | PNPase |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp762K1914 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, PNPT1 antibody, 3'-5' RNA exonuclease OLD35 antibody, polynucleotide phosphorylase-like protein antibody, PNPase 1 antibody, PNPase old-35 antibody, "polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody", polynucleotide phosphorylase 1 antibody, polyribonucleotide nucleotidyltransferase 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
86 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (Uniprot ID# Q8TCS8)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase(107-11174) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2500.
PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis.
Sample: Paraffin-embedded human normal colon.
PNPase antibody (107-11174) dilution: 1:250.
Sample: Paraffin-embedded human normal colon.
PNPase antibody (107-11174) dilution: 1:250.
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-11174 diluted at 1:1000
A: A431
7.5% SDS PAGE
107-11174 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.