Synonyms
MGC138367 antibody, MGC138369 antibody, RPB19 antibody, RPB7 antibody, hRPB19 antibody, hsRPB7 antibody, POLR2G antibody, RNA polymerase II 19 kDa subunit antibody, DNA-directed RNA polymerase II 19 kDa polypeptide antibody, DNA-directed RNA polymerase II subunit RPB7 antibody, RNA polymerase II subunit B7 antibody, DNA directed RNA polymerase II 19 kda polypeptide antibody, DNA-directed RNA polymerase II subunit G antibody, RNA polymerase II seventh subunit antibody, polymerase (RNA) II (DNA directed) polypeptide G antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
19 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 122 (P62487) of RNA polymerase IIG
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using POLR2G(107-10802) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: A431
12% SDS PAGE
107-10802 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.