POLR3A antibody, 0.89 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 11128 |
| Protein Name / Synonyms | POLR3A |
| Concentration (lot specific) | 0.89 mg/ml |
| Recommended Applications | ICC/IF, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
RPC1 antibody, RPC155 antibody, hRPC155 antibody, POLR3A antibody, DNA-directed RNA polymerase III subunit RPC1 antibody, RNA polymerase III subunit C160 antibody, DNA-directed RNA polymerase III subunit A antibody, RNA polymerase III subunit RPC155-D antibody, RNA polymerase III 155 kDa subunit antibody, DNA-directed RNA polymerase III largest subunit antibody, RNA polymerase III subunit C1 antibody, "polymerase (RNA) III (DNA directed) polypeptide A, 155kDa antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
156 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (Uniprot ID# O14802)
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using POLR3A(107-10593) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.
Sample (30 ug of whole cell lysate)
A: HCT116
5% SDS PAGE
107-10593 diluted at 1:500
A: HCT116
5% SDS PAGE
107-10593 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.