Rabbit Anti-Human POU2AF1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16633 |
| Gene Id | 5450 |
| Gene Symbols | POU2AF1 |
| Protein Name / Synonyms | POU2AF1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This POU2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-27 amino acids from the N-terminal region of human POU2AF1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Specificity
Other Names
POU2AF1; OBF1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1
NCBI Accession #
NP_006226.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
27436
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IHC: 1:10-50
 | Western blot analysis of POU2AF1 Antibody (N-term) (Cat# 102-18141) in Ramos cell line lysates (35ug/lane). POU2AF1 (arrow) was detected using the purified Pab. |
 | POU2AF1 Antibody (N-term) (Cat# 102-18141) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | POU2AF1 Antibody (N-term) (Cat# 102-18141)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POU2AF1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.