PPA1 antibody, 0.93 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5464 |
| Protein Name / Synonyms | PPA1 |
| Concentration (lot specific) | 0.93 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
IOPPP antibody, MGC111556 antibody, PP antibody, PP1 antibody, SID6-8061 antibody, PPA1 antibody, pyrophosphate phospho-hydrolase antibody, cytosolic inorganic pyrophosphatase antibody, inorganic pyrophosphatase 1 antibody, inorganic diphosphatase antibody, pyrophosphatase 1 antibody, inorganic pyrophosphatase antibody, diphosphate phosphohydrolase antibody, PPase antibody, pyrophosphatase (inorganic) 1 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 289 of PPA1 (Uniprot ID# Q15181)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PPA1 antibody detects PPA1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000
PPA1 antibody detects PPA1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
PPA1 antibody (107-10946) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1(107-10946) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PPA1(107-10946) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2
D: Molt-4
12% SDS PAGE
107-10946 diluted at 1:10000
A: H1299
B: Hela
C: Hep G2
D: Molt-4
12% SDS PAGE
107-10946 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.