Synonyms
MGC150606 antibody, MGC71074 antibody, PAK2 antibody, PRK2 antibody, PRKCL2 antibody, PRO2042 antibody, Pak-2 antibody, PKN2 antibody, protein-kinase C-related kinase 2 antibody, cardiolipin-activated protein kinase Pak2 antibody, protein kinase C-like 2 antibody, serine/threonine-protein kinase N2 antibody, protein kinase N2 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
112 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 46 and 366 of PRK2 (Uniprot ID# Q16513)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

PRK2 antibody [N1N3] detects PRK2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
PRK2 antibody [N1N3] (107-10976) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using protein kinase N2(107-10976) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
107-10976 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.