PRKCSH antibody [N1C1], 0.94 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5589 |
| Protein Name / Synonyms | PRKCSH |
| Concentration (lot specific) | 0.94 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AGE-R2 antibody, G19P1 antibody, PCLD antibody, PLD1 antibody, PRKCSH antibody, hepatocystin antibody, glucosidase 2 subunit beta antibody, AGE-binding receptor 2 antibody, "glucosidase II, beta subunit antibody", glucosidase II subunit beta antibody, protein kinase C substrate 60.1 kDa protein heavy chain antibody, "protein kinase C substrate, 80 Kda protein antibody", protein kinase C substrate 80K-H antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
59 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 283 of PRKCSH (Uniprot ID# P14314)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:5000-1:20000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PRKCSH antibody [N1C1] detects PRKCSH protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRKCSH protein stained by PRKCSH antibody [N1C1] (107-10299) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PRKCSH protein stained by PRKCSH antibody [N1C1] (107-10299) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PRKCSH(107-10299) antibody at 1:200 dilution.
PRKCSH antibody [N1C1] detects PRKCSH protein at cytoplasm on normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded normal liver.
PRKCSH antibody [N1C1] (107-10299) dilution: 1:500.
Sample: Paraffin-embedded normal liver.
PRKCSH antibody [N1C1] (107-10299) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-10299 diluted at 1:10000
A: A431
7.5% SDS PAGE
107-10299 diluted at 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.