This PRMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-347 amino acids from the C-terminal region of human PRMT1.
Host Species
Rabbit
Clonality
Polyclonal
Species
Human
Introduction
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
PRMT1; HMT2; HRMT1L2; IR1B4; Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; Interferon receptor 1-bound protein 4
NCBI Accession #
NP_001527.3;NP_938074.2
Other Accession #
Q63009; Q9JIF0
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
41516
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
Western blot analysis of PRMT1 (arrow) using rabbit polyclonal PRMT1 Antibody (C-term) (RB00005). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRMT1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
