Profilin 2 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5217 |
| Protein Name / Synonyms | Profilin 2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
D3S1319E antibody, PFL antibody, PFN2 antibody, profilin II antibody, profilin-2 antibody, profilin 2 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
15 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 90 (P35080) of Profilin 2
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Profilin 2 antibody detects PFN2 protein at cytoplasm on colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
Profilin 2 antibody (107-10780) dilution: 1:500.
Sample: Paraffin-embedded colon carcinoma.
Profilin 2 antibody (107-10780) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
107-10780 diluted at 1:1000
A: mouse brain
15% SDS PAGE
107-10780 diluted at 1:1000
Sample(30 ug whole cell lysate)
A: A431
B: H1299
12% SDS PAGE
107-10780 diluted at 1:500
A: A431
B: H1299
12% SDS PAGE
107-10780 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.