Protease Inhibitor 15 antibody [C2C3], C-Terminus, 0.6 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 51050 |
| Protein Name / Synonyms | Protease Inhibitor 15, C-term |
| Concentration (lot specific) | 0.6 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CRISP8 antibody, DKFZp686F0366 antibody, P24TI antibody, P25TI antibody, PI15 antibody, 25 kDa trypsin inhibitor antibody, sugarCrisp antibody, protease inhibitor 15 antibody, CRISP-8 antibody, PI-15 antibody, cysteine-rich secretory protein 8 antibody, peptidase inhibitor 15 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 195 and 258 of Protease Inhibitor 15 (Uniprot ID# O43692)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Protease Inhibitor 15(107-10724) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Breast ca, using Protease Inhibitor 15(107-10724) antibody at 1:250 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
Protease Inhibitor 15 antibody
107-10724 diluted at 1:1000
A: Hela
12% SDS PAGE
Protease Inhibitor 15 antibody
107-10724 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.