Proteasome 20S alpha 2 antibody [N1C3], 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5683 |
| Protein Name / Synonyms | Proteasome 20S alpha 2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
HC3 antibody, MU antibody, PMSA2 antibody, PSC2 antibody, PSMA2 antibody, proteasome component C3 antibody, proteasome subunit alpha type-2 antibody, multicatalytic endopeptidase complex subunit C3 antibody, macropain subunit C3 antibody, proteasome subunit HC3 antibody, "proteasome (prosome, macropain) subunit, alpha type, 2 antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
26 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 234 of Proteasome 20S alpha 2 (Uniprot ID# P25787)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using Proteasome 20S alpha 2(107-10393) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha2(107-10393) antibody at 1:200 dilution.
Sample(30µg of whole cell lysate)
A: H1299
B: HeLa S3
C: Hep G2
D: MOLT4
E: Raji
12% SDS PAGE
107-10393 diluted at 1:1000
A: H1299
B: HeLa S3
C: Hep G2
D: MOLT4
E: Raji
12% SDS PAGE
107-10393 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.