Proteasome 20S alpha 6 antibody, 1 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 5687 |
| Protein Name / Synonyms | Proteasome 20S alpha 6 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
IOTA antibody, MGC22756 antibody, MGC2333 antibody, MGC23846 antibody, PROS27 antibody, p27K antibody, PSMA6 antibody, 27 kDa prosomal protein antibody, multicatalytic endopeptidase complex iota chain antibody, macropain iota chain antibody, prosomal P27K protein antibody, proteasome subunit alpha type-6 antibody, PROS-27 antibody, macropain subunit iota antibody, proteasome iota chain antibody, proteasome subunit iota antibody, proteasome (prosome, macropain) subunit, alpha type, 6 antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
27 kDa
Immunogen
Recombinant fragment contain a sequence corresponding to a region within amino acids 57 and 274 of Proteasome 20S alpha 6
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000
A. 50 µg mouse liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000
A. 50 µg rat liver lysate/extract
12 % SDS-PAGE
Proteasome 20S alpha 6 antibody (107-11073) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 (107-11073) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 (107-11073) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: H1299
D: Hela
12% SDS PAGE
107-11073 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: Hela
12% SDS PAGE
107-11073 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.