Rabbit Anti-Human Protein Phosphatase 1 beta (PPP1CB) (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P62140 |
| Gene Id | 5500 |
| Gene Symbols | PPP1CB |
| Protein Name / Synonyms | Protein Phosphatase 1 beta (PPP1CB) (C-term) |
| Clonality | Polyclonal |
| Preparation | This Protein Phosphatase 1 beta (PPP1CB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human Protein Phosphatase 1 beta (PPP1CB). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Protein phosphatase-1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases involved in the dephosphorylation of a variety of proteins. These enzymes work in opposition to the protein kinases to control the level of phosphorylation. Protein phosphatase (PP1) is essential for cell division, and it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis, as well as in regulation of ionic conductances and long-term synaptic plasticity. PP1 has 3 catalytic subunits, designated alpha (PPP1CA), beta (PPP1CB), and gamma (PPP1CC).
Specificity
Predicted Reactivity
Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Xenopus
Other Names
PPP1CB; Serine/threonine-protein phosphatase PP1-beta catalytic subunit
NCBI Accession #
NP_002700.1;NP_996759.1
Other Accession #
Q6GQL2; P62142; P62143; P61292; P62141; P62207; Q3SWW9
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
37187
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of U251 cell stained with PPP1CB Antibody (C-term)(Cat# 102-17870).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PPP1CB primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).PPP1CB immunoreactivity is localized to Cytoplasm and Nucleus significantly. |
 | Western blot analysis of PPP1CB (arrow) using rabbit polyclonal PPP1CB Antibody (K301) (Cat# 102-17870). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPP1CB gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.