PSME3 antibody, 0.65 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 10197 |
| Protein Name / Synonyms | PSME3 |
| Concentration (lot specific) | 0.65 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
Ki antibody, PA28-gamma antibody, PA28G antibody, REG-GAMMA antibody, PSME3 antibody, Ki nuclear autoantigen antibody, proteasome activator 28 subunit gamma antibody, PA28gamma antibody, proteasome activator 28-gamma antibody, 11S regulator complex subunit gamma antibody, 11S regulator complex gamma subunit antibody, activator of multicatalytic protease subunit 3 antibody, Ki antigen antibody, proteasome activator complex subunit 3 antibody, "proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
30 kDa
Immunogen
Recombinant fragment contain a sequence corresponding to a region within amino acids 1 and 254 of PSME3
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSME3(107-10641) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
107-10641 diluted at 1:2000
A: A549
B: H1299
C: HCT116
D: MCF-7
12% SDS PAGE
107-10641 diluted at 1:2000
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
12% SDS PAGE
107-10641 diluted at 1:2000
A: NIH-3T3
B: JC
C: BCL-1
12% SDS PAGE
107-10641 diluted at 1:2000
Sample (30 ug of whole cell lysate)
A: PC-12
12% SDS PAGE
107-10641 diluted at 1:2000
A: PC-12
12% SDS PAGE
107-10641 diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using PSME3(107-10641) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.