Rabbit Anti-Human PTK2 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q05397 |
| Gene Id | 5747 |
| Gene Symbols | PTK2 |
| Protein Name / Synonyms | PTK2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 821-848 amino acids from the C-terminal region of human PTK2. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
PTK2; FAK; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
NCBI Accession #
NP_001186578.1;NP_722560.1
Other Accession #
O35346; P34152
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
119233
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of PTK2 (arrow) using rabbit polyclonal PTK2 Antibody (C-term) (Cat# 102-17946). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK2 gene (Lane 2). |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | PTK2 Antibody (C-term) (Cat# 102-17946) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of PTK2 Antibody (C-term)(Cat# 102-17946) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.