Pyruvate Kinase (liver/RBC) antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 5313 |
| Protein Name / Synonyms | Pyruvate Kinase (liver/RBC) |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Phosphorylation, Energy Metabolism, Glycolysis & Lipid Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
PK1 antibody, PKL antibody, PKR antibody, PKRL antibody, RPK antibody, PKLR antibody, R-type/L-type pyruvate kinase antibody, pyruvate kinase type L antibody, red cell/liver pyruvate kinase antibody, pyruvate kinase 1 antibody, pyruvate kinase isozyme R/L antibody, "pyruvate kinase, liver and blood cell antibody", pyruvate kinase isozymes R/L antibody, "pyruvate kinase, liver and RBC antibody"
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
62 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 260 of Pyruvate Kinase (liver/RBC) (Uniprot ID# P30613)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Pyruvate Kinase (liver/RBC) antibody detects PKLR protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody (107-10974) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HepG2 cells were fixed in -20? 100% MeOH for 5 min.
Green: PKLR protein stained by Pyruvate Kinase (liver/RBC) antibody (107-10974) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Pyruvate Kinase (liver/RBC)(107-10974) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
E: A375
7.5% SDS PAGE
107-10974 diluted at 1:1000
A: 293T
B: A431
C: HeLa
D: HepG2
E: A375
7.5% SDS PAGE
107-10974 diluted at 1:1000
Pyruvate Kinase (liver/RBC) antibody detects Pyruvate Kinase (liver/RBC) protein by Western blot analysis.
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (107-10974) dilution: 1:10000
A. 20 ug Rat liver lysate/extract
7.5% SDS-PAGE
Pyruvate Kinase (liver/RBC) antibody (107-10974) dilution: 1:10000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.